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| VLS | Virgin Lip Syndrome | |
| VLS | VisionPlus Loyalty System | |
| VLS | Vandenberg Launch and Landing Site | |
| VLS | Virtual Ligand Screening | |
| VLS | Verification of Lawful Status | |
| VLS | Vermont Law School | |
| VLS | Volunteer Legal Services | |
| VLS | Vehicle Lighting Solutions | |
| VLS | Visual Learning Systems | |
| VLS | Visual Listing Systems | |
| VLS | Villanova Law School | |
| VLS | Volume Level Scaling | |
| VLS | Vascular Leak Syndrome | |
| VLS | Virtual Library System | |
| VLS | Valk Last Slot | |
| VLS | Vapour Liquid Solid | |
| VLS | Velocity Lowest Selectable | |
| VLS | Very Low and Strong | |
| VLS | Variable Launch and Spin | |
| VLS | Visionary Link Service | |
| VLS | Vanguard Logistics Services | |
| VLS | Virtual Learning Solutions | |
| VLS | Virtual Lab School | |
| VLS | Vacuum Lifting Systems | |
| VLS | Ventro-Lateral Septum |
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